基因
寡核苷酸池
新一代测序 (NGS)
基因突变文库
抗体
IVDR 解决方案
Twist Bioscience HQ
681 Gateway Blvd
South San Francisco, CA 94080
Reliable, scalable cfDNA workflows
Generate high-quality libraries from challenging cfDNA inputs with a streamlined workflow that delivers high conversion and sensitivity for rare variant detection. Designed for low input and fragmented samples, the Twist cfDNA Library Prep Kit helps you uncover critical insights with confidence and efficiency.
最大限度地检测罕见变异
高转换率能够可靠地检测出罕见变异 (≤0.1% VAF)。Support duplex sequencing workflows with the Twist UMI Adapter System to maximize detection sensitivity.
Streamlined workflow for precious samples
Achieve robust performance, even with low (<1 ng) sample input. Get sequence-ready libraries in 2 hours.
无偏倚扩增
Get consistent coverage across 5%–95% GC, preserving challenging genomic regions and difficult-to-capture targets. Get consistent high yields from complex libraries, even at ≤100 pg of DNA input. Call variants with confidence.
产品数据
High-fidelity amplification with Twist TrueAmp Polymerase generates cfDNA libraries that have substitution error rates comparable to those of PCR-free cfDNA libraries across all analyzed base changes.
Figure 1 Comparable Base Substitution Error Rates with and without TrueAmp Amplification. The Twist cfDNA Library Prep Kit was used to generate libraries with the Twist cfDNA Pan-Cancer Reference Standard v2 as the input material. Twist Full-Length Unique Dual Index (UDI) Adapters were used during ligation for the PCR-free condition and Twist Universal Adapters were used during ligation for the PCR condition. PCR 处理组使用 Twist TrueAmp 聚合酶预混液和 UDI 引物进行 6 个循环的扩增。对文库进行合并、测序和分析。结果显示无 PCR 文库与 TrueAmp 扩增文库的错误率相当。
The Twist cfDNA Library Prep Kit delivers higher cfDNA library yields. Following library generation, samples were eluted in 20 μL of water, and 1 µL was quantified with Qubit™ dsDNA Broad Range Kit.
图 2. The Twist cfDNA Library Prep Kit delivers higher cfDNA library yields. Following library generation, samples were eluted in 20 uL of water, and 1 uL was quantified with Qubit™ dsDNA Broad Range Kit.
The Twist cfDNA Library Prep Kit enables sensitive, accurate detection down to 0.25% VAF. Single-plex capture with a 50 kb oncology panel targets 217 single nucleotide polymorphisms (SNPs). 遗失变异体是指在可检测到的位点总数中，变异体家族 <1 的位点。
图 3. The Twist cfDNA Library Prep Kit enables sensitive and accurate detection of variants at 0.25% VAF. 用定制的 50kb 肿瘤学组合（靶向泛癌种标准品中的 217 个 SNP 变异位点）捕获单重文库。变异体遗失的计算方法是将变异体家族少于 1 个的位点总和除以可检测到的变异体位点总数。
The Twist cfDNA Library Prep Kit delivers higher library complexity with or without unique molecular identifier (UMI) deduplication. Single-plex capture with a 50 kb oncology panel targets variant sites in cfDNA standard material using optimized target enrichment workflows.
图 4. The Twist cfDNA Library Prep Kit delivers higher complexity with and without UMI deduplication.
视频
High-fidelity amplification with PCR
High-fidelity amplification with Twist TrueAmp Polymerase generates cfDNA libraries that have substitution error rates comparable to those of PCR-free cfDNA libraries across all analyzed base changes.
Figure 1 Comparable Base Substitution Error Rates with and without TrueAmp Amplification. The Twist cfDNA Library Prep Kit was used to generate libraries with the Twist cfDNA Pan-Cancer Reference Standard v2 as the input material. Twist Full-Length Unique Dual Index (UDI) Adapters were used during ligation for the PCR-free condition and Twist Universal Adapters were used during ligation for the PCR condition. PCR 处理组使用 Twist TrueAmp 聚合酶预混液和 UDI 引物进行 6 个循环的扩增。对文库进行合并、测序和分析。结果显示无 PCR 文库与 TrueAmp 扩增文库的错误率相当。
更高的转换率 = 更高的收益率
The Twist cfDNA Library Prep Kit delivers higher cfDNA library yields. Following library generation, samples were eluted in 20 μL of water, and 1 µL was quantified with Qubit™ dsDNA Broad Range Kit.
图 2. The Twist cfDNA Library Prep Kit delivers higher cfDNA library yields. Following library generation, samples were eluted in 20 uL of water, and 1 uL was quantified with Qubit™ dsDNA Broad Range Kit.
更高的灵敏度，更高的精度
The Twist cfDNA Library Prep Kit enables sensitive, accurate detection down to 0.25% VAF. Single-plex capture with a 50 kb oncology panel targets 217 single nucleotide polymorphisms (SNPs). 遗失变异体是指在可检测到的位点总数中，变异体家族 <1 的位点。
图 3. The Twist cfDNA Library Prep Kit enables sensitive and accurate detection of variants at 0.25% VAF. 用定制的 50kb 肿瘤学组合（靶向泛癌种标准品中的 217 个 SNP 变异位点）捕获单重文库。变异体遗失的计算方法是将变异体家族少于 1 个的位点总和除以可检测到的变异体位点总数。
改进的双工功能 = 更高的复杂性
The Twist cfDNA Library Prep Kit delivers higher library complexity with or without unique molecular identifier (UMI) deduplication. Single-plex capture with a 50 kb oncology panel targets variant sites in cfDNA standard material using optimized target enrichment workflows.
图 4. The Twist cfDNA Library Prep Kit delivers higher complexity with and without UMI deduplication.
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High-fidelity amplification with PCR
High-fidelity amplification with Twist TrueAmp Polymerase generates cfDNA libraries that have substitution error rates comparable to those of PCR-free cfDNA libraries across all analyzed base changes.
更高的转换率 = 更高的收益率
The Twist cfDNA Library Prep Kit delivers higher cfDNA library yields. Following library generation, samples were eluted in 20 μL of water, and 1 µL was quantified with Qubit™ dsDNA Broad Range Kit.
更高的灵敏度，更高的精度
The Twist cfDNA Library Prep Kit enables sensitive, accurate detection down to 0.25% VAF. Single-plex capture with a 50 kb oncology panel targets 217 single nucleotide polymorphisms (SNPs). 遗失变异体是指在可检测到的位点总数中，变异体家族 <1 的位点。
改进的双工功能 = 更高的复杂性
The Twist cfDNA Library Prep Kit delivers higher library complexity with or without unique molecular identifier (UMI) deduplication. Single-plex capture with a 50 kb oncology panel targets variant sites in cfDNA standard material using optimized target enrichment workflows.
