Genes
Grupos de oligonucleótidos
NGS
Bibliotecas de variantes
Anticuerpos
Soluciones compatibles con las IVDR
Twist Bioscience HQ
681 Gateway Blvd
South San Francisco, CA 94080
Reliable, scalable cfDNA workflows
Generate high-quality libraries from challenging cfDNA inputs with a streamlined workflow that delivers high conversion and sensitivity for rare variant detection. Designed for low input and fragmented samples, the Twist cfDNA Library Prep Kit helps you uncover critical insights with confidence and efficiency.
Maximice la detección de variantes infrecuentes
La alta tasa de conversión detecta con fiabilidad las variantes infrecuentes (≤0,1 % VAF) Support duplex sequencing workflows with the Twist UMI Adapter System to maximize detection sensitivity.
Streamlined workflow for precious samples
Achieve robust performance, even with low (<1 ng) sample input. Get sequence-ready libraries in 2 hours.
Amplificación sin sesgo
Get consistent coverage across 5%–95% GC, preserving challenging genomic regions and difficult-to-capture targets. Get consistent high yields from complex libraries, even at ≤100 pg of DNA input. Call variants with confidence.
Datos del producto
High-fidelity amplification with Twist TrueAmp Polymerase generates cfDNA libraries that have substitution error rates comparable to those of PCR-free cfDNA libraries across all analyzed base changes.
Figure 1 Comparable Base Substitution Error Rates with and without TrueAmp Amplification. The Twist cfDNA Library Prep Kit was used to generate libraries with the Twist cfDNA Pan-Cancer Reference Standard v2 as the input material. Twist Full-Length Unique Dual Index (UDI) Adapters were used during ligation for the PCR-free condition and Twist Universal Adapters were used during ligation for the PCR condition. La condición de PCR se amplificó utilizando 6 ciclos con mezcla de polimerasa TrueAmp Twist y cebadores de UDI. Las bibliotecas se agruparon, secuenciaron y analizaron. Se observan tasas de error comparables entre las bibliotecas sin PCR y TrueAmp.
The Twist cfDNA Library Prep Kit delivers higher cfDNA library yields. Following library generation, samples were eluted in 20 μL of water, and 1 µL was quantified with Qubit™ dsDNA Broad Range Kit.
Figura 2. The Twist cfDNA Library Prep Kit delivers higher cfDNA library yields. Following library generation, samples were eluted in 20 uL of water, and 1 uL was quantified with Qubit™ dsDNA Broad Range Kit.
The Twist cfDNA Library Prep Kit enables sensitive, accurate detection down to 0.25% VAF. Single-plex capture with a 50 kb oncology panel targets 217 single nucleotide polymorphisms (SNPs). Las variantes omitidas son sitios con <1 familia de variantes sobre el total de sitios detectables.
Figura 3. The Twist cfDNA Library Prep Kit enables sensitive and accurate detection of variants at 0.25% VAF. Las bibliotecas se capturaron monoplexadas con un panel oncológico personalizado de 50 kb dirigido a los sitios de variantes de 217 SNP en el material estándar pantumoral. Las variantes ausentes se calculan sumando los sitios con menos de 1 familia de variantes y dividiendo el resultado entre el número de sitios de variantes detectables totales.
The Twist cfDNA Library Prep Kit delivers higher library complexity with or without unique molecular identifier (UMI) deduplication. Single-plex capture with a 50 kb oncology panel targets variant sites in cfDNA standard material using optimized target enrichment workflows.
Figura 4. The Twist cfDNA Library Prep Kit delivers higher complexity with and without UMI deduplication.
Vídeo
High-fidelity amplification with PCR
High-fidelity amplification with Twist TrueAmp Polymerase generates cfDNA libraries that have substitution error rates comparable to those of PCR-free cfDNA libraries across all analyzed base changes.
Figure 1 Comparable Base Substitution Error Rates with and without TrueAmp Amplification. The Twist cfDNA Library Prep Kit was used to generate libraries with the Twist cfDNA Pan-Cancer Reference Standard v2 as the input material. Twist Full-Length Unique Dual Index (UDI) Adapters were used during ligation for the PCR-free condition and Twist Universal Adapters were used during ligation for the PCR condition. La condición de PCR se amplificó utilizando 6 ciclos con mezcla de polimerasa TrueAmp Twist y cebadores de UDI. Las bibliotecas se agruparon, secuenciaron y analizaron. Se observan tasas de error comparables entre las bibliotecas sin PCR y TrueAmp.
Tasa de conversión más alta = rendimiento más alto
The Twist cfDNA Library Prep Kit delivers higher cfDNA library yields. Following library generation, samples were eluted in 20 μL of water, and 1 µL was quantified with Qubit™ dsDNA Broad Range Kit.
Figura 2. The Twist cfDNA Library Prep Kit delivers higher cfDNA library yields. Following library generation, samples were eluted in 20 uL of water, and 1 uL was quantified with Qubit™ dsDNA Broad Range Kit.
Mayor sensibilidad y mayor precisión
The Twist cfDNA Library Prep Kit enables sensitive, accurate detection down to 0.25% VAF. Single-plex capture with a 50 kb oncology panel targets 217 single nucleotide polymorphisms (SNPs). Las variantes omitidas son sitios con <1 familia de variantes sobre el total de sitios detectables.
Figura 3. The Twist cfDNA Library Prep Kit enables sensitive and accurate detection of variants at 0.25% VAF. Las bibliotecas se capturaron monoplexadas con un panel oncológico personalizado de 50 kb dirigido a los sitios de variantes de 217 SNP en el material estándar pantumoral. Las variantes ausentes se calculan sumando los sitios con menos de 1 familia de variantes y dividiendo el resultado entre el número de sitios de variantes detectables totales.
Duplexión mejorada = mayor complejidad
The Twist cfDNA Library Prep Kit delivers higher library complexity with or without unique molecular identifier (UMI) deduplication. Single-plex capture with a 50 kb oncology panel targets variant sites in cfDNA standard material using optimized target enrichment workflows.
Figura 4. The Twist cfDNA Library Prep Kit delivers higher complexity with and without UMI deduplication.
1/5
High-fidelity amplification with PCR
High-fidelity amplification with Twist TrueAmp Polymerase generates cfDNA libraries that have substitution error rates comparable to those of PCR-free cfDNA libraries across all analyzed base changes.
Tasa de conversión más alta = rendimiento más alto
The Twist cfDNA Library Prep Kit delivers higher cfDNA library yields. Following library generation, samples were eluted in 20 μL of water, and 1 µL was quantified with Qubit™ dsDNA Broad Range Kit.
Mayor sensibilidad y mayor precisión
The Twist cfDNA Library Prep Kit enables sensitive, accurate detection down to 0.25% VAF. Single-plex capture with a 50 kb oncology panel targets 217 single nucleotide polymorphisms (SNPs). Las variantes omitidas son sitios con <1 familia de variantes sobre el total de sitios detectables.
Duplexión mejorada = mayor complejidad
The Twist cfDNA Library Prep Kit delivers higher library complexity with or without unique molecular identifier (UMI) deduplication. Single-plex capture with a 50 kb oncology panel targets variant sites in cfDNA standard material using optimized target enrichment workflows.
