Cell-free DNA Library Prep Kit

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Reliable, scalable cfDNA workflows

Generate high-quality libraries from challenging cfDNA inputs with a streamlined workflow that delivers high conversion and sensitivity for rare variant detection. Designed for low input and fragmented samples, the Twist cfDNA Library Prep Kit helps you uncover critical insights with confidence and efficiency.

レアバリアントの検出を最大化

高変換効率のため、レアバリアント（≦ 0.1% VAF）を確実に検出。Support duplex sequencing workflows with the Twist UMI Adapter System to maximize detection sensitivity.

Streamlined workflow for precious samples

Achieve robust performance, even with low (<1 ng) sample input. Get sequence-ready libraries in 2 hours.

バイアスのない増幅

Get consistent coverage across 5%–95% GC, preserving challenging genomic regions and difficult-to-capture targets. Get consistent high yields from complex libraries, even at ≤100 pg of DNA input. Call variants with confidence.

製品データ

High-fidelity amplification with Twist TrueAmp Polymerase generates cfDNA libraries that have substitution error rates comparable to those of PCR-free cfDNA libraries across all analyzed base changes.

The Twist cfDNA Library Prep Kit delivers higher cfDNA library yields. Following library generation, samples were eluted in 20 μL of water, and 1 µL was quantified with Qubit™ dsDNA Broad Range Kit.

The Twist cfDNA Library Prep Kit enables sensitive, accurate detection down to 0.25% VAF. Single-plex capture with a 50 kb oncology panel targets 217 single nucleotide polymorphisms (SNPs). 未検出バリアントとは、検出可能な全部位数に対してバリアントファミリーが 1 つ未満の部位を指します。

The Twist cfDNA Library Prep Kit delivers higher library complexity with or without unique molecular identifier (UMI) deduplication. Single-plex capture with a 50 kb oncology panel targets variant sites in cfDNA standard material using optimized target enrichment workflows.

ビデオ

cfDNA sequencing error rate comparison showing PCR-free vs TrueAmp amplification libraries with equivalent base substitution accuracy for low-frequency variant detection

cfDNA library preparation kit generating higher DNA yield measured by Qubit™, improving NGS library prep efficiency and sequencing performance

Sensitive detection of low-frequency variants at 0.25% VAF using cfDNA library prep and hybridization capture with oncology SNP panel

High-complexity cfDNA sequencing libraries with and without UMI deduplication, improving unique read depth and variant detection accuracy

High-fidelity amplification with PCR

Figure 1 Comparable Base Substitution Error Rates with and without TrueAmp Amplification. The Twist cfDNA Library Prep Kit was used to generate libraries with the Twist cfDNA Pan-Cancer Reference Standard v2 as the input material. Twist Full-Length Unique Dual Index (UDI) Adapters were used during ligation for the PCR-free condition and Twist Universal Adapters were used during ligation for the PCR condition. PCR 条件としては、Twist TrueAmp Polymerase Mix および UDI プライマーを用いて、6 サイクルで増幅を行った。ライブラリをプールし、シーケンシングを行って分析した。PCR なしで構築したライブラリと TrueAmp を用いたライブラリの間で、同程度のエラー率が見られる。

高変換率 = 高収量

図 2. The Twist cfDNA Library Prep Kit delivers higher cfDNA library yields. Following library generation, samples were eluted in 20 uL of water, and 1 uL was quantified with Qubit™ dsDNA Broad Range Kit.

より高感度かつ高精度

図 3. The Twist cfDNA Library Prep Kit enables sensitive and accurate detection of variants at 0.25% VAF. ライブラリは、Pan-Cancer Standard サンプルにおける 217 個の SNP 変異部位を標的とするカスタム 50 kb オンコロジーパネルを用いてシングルプレックスでキャプチャ。「Variant Missed」（検出されなかったバリアント率）は、バリアントファミリーが 1 つ未満の部位の合計を、検出可能なバリアント部位の総数で割ることで算出されます。

重複率の改善 = 複雑性の高さ

図 4. The Twist cfDNA Library Prep Kit delivers higher complexity with and without UMI deduplication.

High-fidelity amplification with PCR

高変換率 = 高収量

より高感度かつ高精度

重複率の改善 = 複雑性の高さ

See how the Twist cfDNA Library Prep Kit and Ultima Genomics ppmSeq® technology work together to maximize conversion of fragmented cfDNA into high-quality sequencing libraries. Learn how this PCR-free workflow improves confidence in ultra-low-frequency variant detection while simplifying library preparation and reducing sequencing costs for whole genome sequencing applications.

The cfDNA Library Prep Kit with TrueAmp Polymerase Mix workflow

The cfDNA workflow leverages engineered GC balance and high-fidelity amplification. This design delivers high amplification yield while minimizing PCR-introduced errors that can compromise low-frequency variant detection. その結果、2 時間という高速ワークフローで、均一かつ高品質なライブラリが得られます。アプタマーベースのホットスタート制御により、自動化ワークフローに適した室温における安定性を実現し、単一チューブに2X マスターミックスを入れたことで、WGS およびターゲットエンリッチメントの両方のワークフローを効率化します。

cfDNA NGS workflow with optimized GC balance and high-fidelity PCR amplification delivering uniform libraries, reduced sequencing errors, and improved low-frequency variant detection

Optimize detection in liquid biopsy workflows

Maximize rare variant sensitivity by integrating this kit into your liquid biopsy pipeline. Engineered for low-input samples, this workflow enables the high conversion efficiency required to identify low-frequency mutations with confidence.
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Scale cfDNA whole genome sequencing studies with high fidelity

Maximize the detection of fragmented DNA in WGS. Use low-bias amplification to get uniform genome-wide coverage needed for comprehensive liquid biopsy research and large-scale genomic monitoring of cell-free samples.
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Enhance target enrichment sensitivity for cfDNA

Integrate this kit into your target enrichment workflow to improve the capture of rare variants from limited plasma samples. When you optimize cfDNA conversion before hybridization, the sequenced libraries have high complexity and minimal bias, resulting in deeper target coverage for oncology and prenatal screening applications.
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